背景资料 | |
背景简介 | SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates. |
致瘤性 | Yes, in nude mice; forms malignant melanoma |
抗体表达 | Blood Type O; Rh+; HLA A2, A11, B40, Bw16 |
保藏机构 | ATCC; HTB-70 |
生物安全等级 | BSL-1 |
基本信息 | |
名称 | 皮肤恶性黑色素瘤细胞SK-MEL-5(STR鉴定正确) |
别称 | SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel |
种属 | 人 |
组织来源 | 皮肤 |
细胞类型 | 肿瘤细胞 |
疾病类型 | 皮肤黑色素瘤 |
生长特性 | 贴壁生长 |
细胞形态 | 星状细胞样 |
冻存条件 | 冻存液:90%FBS,DMSO 10%;建议使用非程序冻存液盒货号:LBH-001 |
培养条件 | 生长培养基:MEM培养基;10%胎牛血清;1%双抗 生长气相:空气95%,CO2,5%,;温度:37℃,培养箱湿度:70%-80%。 |
传代比例 细胞消化 | 1:2传代 消化2-3分钟 |
倍增时间 | 每周 2 至 3 次 |
产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |
注意事项 | / |