背景资料 | |
背景简介 | RLE-6TN is an alveolar cell that was isolated from the lung of a 56-day-old male FISCHER 344 (F344) rat. At passage 5, the alveolar Type II cells were transfected with SV40 (pRSV-T DNA) by lipofection. Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization. The cell was deposited by KE Driscoll. |
保藏机构 | ATCC; CRL-2300 |
生物安全等级 | BSL-2 |
基本信息 | |
名称 | 大鼠肺泡Ⅱ型细胞RLE-6TN(种属鉴定) |
别称 | Rat Lung Epithelial-6-T-antigen Negative |
种属 | 大鼠 |
组织来源 | 肺,肺泡细胞,II型 |
细胞类型 | 正常细胞 |
疾病类型 | 转化细胞系 |
生长特性 | 贴壁生长 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:90%FBS,DMSO 10%;建议使用非程序冻存液盒货号:LBH-001 |
培养条件 | 生长培养基:RPMI1640培养基;10%胎牛血清;1%双抗 生长气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 |
传代比例 细胞消化 | 1:2传代 消化1-2分钟 |
倍增时间 | 每周 2 至 3 次 |
产品使用 | 仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |
注意事项 | / |