背景资料 | |
背景简介 | SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
致瘤性 | Yes; Yes, forms tumors in nude mice |
保藏机构 | ATCC; CRL-2149 |
生物安全等级 | BSL-1 |
基本信息 | |
名称 | |
别称 | SKNDZ |
种属 | 人 |
组织来源 | 大脑 |
细胞类型 | 肿瘤细胞 |
疾病类型 | 神经母细胞瘤细胞 |
生长特性 | 贴壁生长 |
细胞形态 | 成神经细胞样 |
冻存条件 | 冻存液：90%FBS，DMSO 10%；建议使用非程序冻存液盒货号：LBH-001 |
培养条件 | 生长培养基:DMEM培养基 ；10%胎牛血清 ；1%双抗 生长气相:空气95%，CO2，5%,；温度：37℃，培养箱湿度：70%-80%。 |
传代比例 细胞消化 | 1:2传代
|
倍增时间 | 每周 2 至 3 次 |
产品使用 | 仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。 |
注意事项 | 该细胞贴壁松散，收货如有大块脱落或全部脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
