背景简介

sNF96.2 is a Schwann cell line that was isolated in 1996 from a 28-year-old, male patient with neurofibromatosis type 1 Nf1.The sNF96.2 cell line was derived from a recurrent mass associated with nerve and diagnosed as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75.

保藏机构

ATCC; CRL-2884

生物安全等级

BSL-1

基本信息

名称

人雪旺细胞 (神经纤维瘤病Ⅰ型)sNF96.2(STR鉴定正确)

别称

SNF96.2; sNF96-2

种属

人

组织来源

施万细胞

疾病类型

1型神经纤维瘤病Nf1

生长特性

贴壁生长

细胞形态

雪旺细胞样

冻存条件

冻存液：90%FBS，DMSO 10%；建议使用非程序冻存液盒货号：LBH-001

培养条件

生长培养基:DMEM培养基 ；10%胎牛血清 ；1%双抗

生长气相:空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。

传代比例

细胞消化

1:2传代

倍增时间

~33 h

产品使用

仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。

注意事项

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